Evaluation of in vitro Cytotoxic Effects of Especifico Pessoa Phytotherapic Tincture on Ehrlich Tumor Cells and Mice Spleen Cells
DOI:
https://doi.org/10.21664/2238-8869.2020v9i1.p458-472Palavras-chave:
Especifico Pessoa, Fitoterápicos, Tumor de Ehrlich, CitotoxicidadeResumo
Especifico Pessoa (EP) é utilizado no envenenamento por serpentes. Estudos comprovam seu uso tradicional e descrevem outras propriedades, como antitumoral. O objetivo deste estudo foi avaliar seu efeito citotóxico em células do Tumor de Ehrlich e esplênicas de camundongos. O ensaio foi realizado pelo método de exclusão do Azul Tripan. A suspensão de células esplênicas foi obtida (n = 2) em RPMI e a tumoral a partir do fluido ascitico de camundongos portadores do tumor (n = 1), ambos a 4 x 106 células mL-1. O EP foi usado puro ou diluído em RPMI (1:2; 1:4). Os resultados são expressos como porcentagem de viabilidade celular e demonstraram que o EP é tóxico para células tumorais, em todas as concentrações (Controle: 96,42 ± 3,40; Puro: 1,55 ± 2,91; 1:2: 4,85 ± 5,04; 1:4: 13,39 ± 5,08), mas atóxico para os esplenócitos na menor diluição (Controle: 72,86 ± 13,79; Puro: 13,52 ± 6,36; 1:2: 41,36 ± 13,51; 1:4: 56,59 ± 8,62). Portanto, os resultados demonstram que o EP tem efeito citotóxico, dependendo da dose e linhagem celular utilizada.
Referências
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Borges, Célio Campos, Megumi Sadahiro, and Maria Cristina dos Santos. 1999. “Aspectos Epidemiológicos e Clínicos Dos Acidentes Ofídicos Ocorridos Nos Municípios Do Estado Do Amazonas.” Revista Da Sociedade Brasileira de Medicina Tropical 32 (6): 637–46. https://doi.org/10.1590/S0037-86821999000600005.
Brasil, Assembleia da República. 2013. Lei N° 71, de 2 de Setembro de 2013. Brasília. https://data.dre.pt/eli/lei/71/2013/09/02/p/dre/pt/html.
Castoldi, Lindsey, Marjorie Assis Golim, Orlando Garcia Ribeiro Filho, Graziela Gorete Romagnoli, Olga Célia Martinez Ibañez, and Ramon Kaneno. 2007. “Enhanced Natural Killer Activity and Production of Pro-Inflammatory Cytokines in Mice Selected for High Acute Inflammatory Response (AIRmax).” Immunology 120 (3): 372–79. https://doi.org/10.1111/j.1365-2567.2006.02513.x.
Engler, Thomas A., Kenneth O. Lynch, Jayachandra P. Reddy, and G. Stuart Gregory. 1993. “Synthetic Pterocarpans with Anti-HIV Activity.” Bioorganic & Medicinal Chemistry Letters 3 (6): 1229–32. https://doi.org/10.1016/S0960-894X(00)80321-2.
Falcão, Maria José C., Yvone Brígido M. Pouliquem, Mary Anne S. Lima, Nilce Viana Gramosa, Letícia V. Costa-Lotufo, Gardênia Carmen G. Militão, Cláudia Pessoa, Manoel Odorico de Moraes, and Edilberto R. Silveira. 2005. “Cytotoxic Flavonoids from Platymiscium Floribundum.” Journal of Natural Products 68 (3): 423–26. https://doi.org/10.1021/np049854d.
Ferreira, Paulo Michel Pinheiro, Daisy Jereissati Barbosa Lima, Bryan Wender Debiasi, Bruno Marques Soares, Kátia da Conceição Machado, Janaina da Costa Noronha, Domingos de Jesus Rodrigues, Adilson Paulo Sinhorin, Cláudia Pessoa, and Gerardo Magela Vieira Júnior. 2013. “Antiproliferative Activity of Rhinella Marina and Rhaebo Guttatus Venom Extracts from Southern Amazon.” Toxicon 72 (September): 43–51. https://doi.org/10.1016/j.toxicon.2013.06.009.
Kaneno, R, LM Fontanari, SA Santos, LC Di Stasi, E. Rodrigues Filho, and AF Eira. 2004. “Effects of Extracts from Brazilian Sun-Mushroom (Agaricus Blazei) on the NK Activity and Lymphoproliferative Responsiveness of Ehrlich Tumor-Bearing Mice.” Food and Chemical Toxicology 42 (6): 909–16. https://doi.org/10.1016/j.fct.2004.01.014.
Martins, MC Gameiro, L Castoldi, GG Romagnoli, FC Lopes, AVFS Pinto, W Loyola, and R Kaneno. 2008. “Polysaccharide-Rich Fraction of Agaricus Brasiliensis Enhances the Candidacidal Activity of Murine Macrophages.” Memorias Do Instituto Oswaldo Cruz 103 (3): 244–50. https://doi.org/10.1590/S0074-02762008005000011.
Martins, MM Marques, SK Bussadori, RA Mesquita-Ferrari, VCS Pavesi, NS Wadt, and KP Fernandes. 2009. “Citotoxicidade in Vitro de Extratos de Arnica Brasileira (Solidago Microglossa) e Arnica Paulista (Porophyllum Ruderale).” ConScientiae Saúde 8 (1): 99–104. https://doi.org/10.5585/conssaude.v8i1.1457.
Máximo, Patrícia, and Ana Lourenço. 1998. “A Pterocarpan from Ulex Parviflorus.” Phytochemistry 48 (2): 359–62. https://doi.org/10.1016/S0031-9422(97)01090-X.
Militão, GC, INF Dantas, C Pessoa, MJC Falcão, ER Silveira, MAS Lima, R Curi, T Lima, MO Moraes, and LV Costa-Lotufo. 2006. “Induction of Apoptosis by Pterocarpans from Platymiscium Floribundum in HL-60 Human Leukemia Cells.” Life Sciences 78 (20): 2409–17. https://doi.org/10.1016/j.lfs.2005.09.044.
Militão, GC, PC Jimenez, DV Wilke, C Pessoa, MJ Falcão, MA Lima, ER Silveira, MO de Moraes, and LV Costa-Lotufo. 2005. “Antimitotic Properties of Pterocarpans Isolated from Platymiscium Floribundum on Sea Urchin Eggs.” Planta Medica 71 (7): 683–85. https://doi.org/10.1055/s-2005-871277.
Militão, GC, SM Pinheiro, INF Dantas, C Pessoa, MO de Moraes, LV Costa-Lotufo, MAS Lima, and ER Silveira. 2007. “Bioassay-Guided Fractionation of Pterocarpans from Roots of Harpalyce Brasiliana Benth.” Bioorganic & Medicinal Chemistry 15 (21): 6687–91. https://doi.org/10.1016/j.bmc.2007.08.011.
Moura, Valéria Mourão de, and Rosa Helena Veras Mourão. 2012. “Aspectos Do Ofidismo No Brasil e Plantas Medicinais Utilizadas Como Complemento à Soroterapia.” Scientia Amazonia, v. 1, N 3: 17–26. http://www.scientia.ufam.edu.br.
Moura, Valéria Mourão de, Rosa Helena Veras Mourão, and Maria Cristina Dos-Santos. 2015. “Acidentes Ofídicos Na Região Norte Do Brasil e o Uso de Espécies Vegetais Como Tratamento Alternativo e Complementar à Soroterapia.” Scientia Amazonia, no. 1: 73–84. https://doi.org/10.19178/Sci.Amazon.v4i1.73-84.
Nakagawa, Masashi, Koji Nakanishi, Laszlo L. Darko, and James A. Vick. 1982. “Structures of Cabenegrins A-I and A-II, Potent Anti-Snake Venoms.” Tetrahedron Letters 23 (38): 3855–58. https://doi.org/10.1016/S0040-4039(00)87726-6.
Oliveira, Ligianne P., Renata C. Pinheiro, Marcelo S. Vieira, José Realino Paula, Maria Teresa F. Bara, and Marize C. Valadares. 2010. “Atividade Citotóxica e Antiangiogênica de Punica Granatum L., Punicaceae.” Brazilian Journal of Pharmacognosy 20 (2): 201–7. https://doi.org/10.1590/S0102-695X2010000200011.
Pierini, S.V., D.A. Warrell, A. De Paulo, and R.D.G. Theakston. 1996. “High Incidence of Bites and Stings by Snakes and Other Animals among Rubber Tappers and Amazonian Indians of the Juruá Valley, Acre State, Brazil.” Toxicon 34 (2): 225–36. https://doi.org/10.1016/0041-0101(95)00125-5.
Pithayanukul, Pimolpan, Pakatip Ruenraroengsak, Rapepol Bavovada, Narumol Pakmanee, Rutt Suttisri, and Suwipa Saen-oon. 2005. “Inhibition of Naja Kaouthia Venom Activities by Plant Polyphenols.” Journal of Ethnopharmacology 97 (3): 527–33. https://doi.org/10.1016/j.jep.2004.12.013.
Reichert, Alana Meira, Carla Brugin Marek, Ana Maria Itinose, Renata Prestes Antonangelo, Cristiane Weirich Lenzi, Rodrigo Suzuki, and Paulino Filho Yassuda. 2014. “Biochemical Alterations Induced by Phytotherapic Tincture with Antiophidic Activity in Male Wistar Rats.” African Journal of Pharmacy and Pharmacology 8 (28): 737–46. https://doi.org/10.5897/AJPP2013.3874.
Rogero, Sizue Ota, Ademar Benévolo Lugão, Tamiko Ichikawa Ikeda, and Áurea Silveira Cruz. 2003. “Teste in Vitro de Citotoxicidade: Estudo Comparativo Entre Duas Metodologias.” Materials Research 6 (3): 317–20. https://doi.org/10.1590/s1516-14392003000300003.
Silva, AJ da, AL Coelho, AB Simas, RA Moraes, DA Pinheiro, FF Fernandes, EZ Arruda, PR Costa, and PA Melo. 2004. “Synthesis and Pharmacological Evaluation of Prenylated and Benzylated Pterocarpans against Snake Venom.” Bioorganic & Medicinal Chemistry Letters 14 (2): 431–35. https://doi.org/10.1016/j.bmcl.2003.10.044.
Silva, Fernanda Coleraus, Juliete Gomes de Lara de Souza, Alana Meira Reichert, Renata Prestes Antonangelo, Rodrigo Suzuki, Ana Maria Itinose, and Carla Brugin Marek. 2015. “Influence of the Alcohol Present in a Phytotherapic Tincture on Male Rat Lipid Profiles and Renal Function.” Evidence-Based Complementary and Alternative Medicine 2015: 1–11. https://doi.org/10.1155/2015/762373.
Silva, Graça Lúcia Da, Maria Iracema Lacerda Machado, Francisco José de Abreu Matos, and Raimundo Braz-Filho. 1999. “A New Isoflavone Isolated from Harpalyce Brasiliana.” Journal of the Brazilian Chemical Society 10 (6): 438–42. https://doi.org/10.1590/S0103-50531999000600003.
Silva, Graça Lúcia Da, Francisco José de Abreu Matos, and Edilberto Rocha Silveira. 1997. “4′-Dehydroxycabenegrin A-I from Roots of Harpalyce Brasiliana.” Phytochemistry 46 (6): 1059–62. https://doi.org/10.1016/S0031-9422(97)00338-5.
Sultana, Sabira, Hafiz Muhammad Asif, Hafiz Muhammad Irfan Nazar, Naveed Akhtar, Jalil Ur. Rehman, and Riaz Ur. Rehman. 2014. “Medicinal Plants Combating Against Cancer - a Green Anticancer Approach.” Asian Pacific Journal of Cancer Prevention 15 (11): 4385–94. https://doi.org/10.7314/APJCP.2014.15.11.4385.
Vieira, Nashira Campos, Laila Salmen Espíndola, Jaime Martins Santana, Maria Leopoldina Veras, Otília Deusdênia Loiola Pessoa, Sávio Moita Pinheiro, Renata Mendonça de Araújo, Mary Anne Sousa Lima, and Edilberto Rocha Silveira. 2008. “Trypanocidal Activity of a New Pterocarpan and Other Secondary Metabolites of Plants from Northeastern Brazil Flora.” Bioorganic & Medicinal Chemistry 16 (4): 1676–82. https://doi.org/10.1016/j.bmc.2007.11.027.
Ximenes, RM, RS Alves, TP Pereira, RM Araújo, ER Silveira, MM Rabello, MZ Hernandes, VC Soares, D Bristot, and CL Pires. 2012. “Harpalycin 2 Inhibits the Enzymatic and Platelet Aggregation Activities of PrTX-III, a D49 Phospholipase A2 from Bothrops Pirajai Venom.” BMC Complementary and Alternative Medicine 12 (1): 1161. https://doi.org/10.1186/1472-6882-12-139.
Ximenes, RM, MM Rabello, RM Araújo, ER Silveira, FHR Fagundes, EBS Diz-Filho, SC Buzzo, et al. 2012. “Inhibition of Neurotoxic Secretory Phospholipases A 2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce Brasiliana Benth.” Evidence-Based Complementary and Alternative Medicine, 1–9. https://doi.org/10.1155/2012/987517.
Araújo, Renata Mendonça, Sávio Moita Pinheiro, Mary Anne Sousa Lima, and Edilberto Rocha Silveira. 2008. “Complete NMR Data Assignments for Novel Pterocarpans from Harpalyce Brasiliana.” Magnetic Resonance in Chemistry 46 (9): 890–93. https://doi.org/10.1002/mrc.2269.
Borges, Célio Campos, Megumi Sadahiro, and Maria Cristina dos Santos. 1999. “Aspectos Epidemiológicos e Clínicos Dos Acidentes Ofídicos Ocorridos Nos Municípios Do Estado Do Amazonas.” Revista Da Sociedade Brasileira de Medicina Tropical 32 (6): 637–46. https://doi.org/10.1590/S0037-86821999000600005.
Brasil, Assembleia da República. 2013. Lei N° 71, de 2 de Setembro de 2013. Brasília. https://data.dre.pt/eli/lei/71/2013/09/02/p/dre/pt/html.
Castoldi, Lindsey, Marjorie Assis Golim, Orlando Garcia Ribeiro Filho, Graziela Gorete Romagnoli, Olga Célia Martinez Ibañez, and Ramon Kaneno. 2007. “Enhanced Natural Killer Activity and Production of Pro-Inflammatory Cytokines in Mice Selected for High Acute Inflammatory Response (AIRmax).” Immunology 120 (3): 372–79. https://doi.org/10.1111/j.1365-2567.2006.02513.x.
Engler, Thomas A., Kenneth O. Lynch, Jayachandra P. Reddy, and G. Stuart Gregory. 1993. “Synthetic Pterocarpans with Anti-HIV Activity.” Bioorganic & Medicinal Chemistry Letters 3 (6): 1229–32. https://doi.org/10.1016/S0960-894X(00)80321-2.
Falcão, Maria José C., Yvone Brígido M. Pouliquem, Mary Anne S. Lima, Nilce Viana Gramosa, Letícia V. Costa-Lotufo, Gardênia Carmen G. Militão, Cláudia Pessoa, Manoel Odorico de Moraes, and Edilberto R. Silveira. 2005. “Cytotoxic Flavonoids from Platymiscium Floribundum.” Journal of Natural Products 68 (3): 423–26. https://doi.org/10.1021/np049854d.
Ferreira, Paulo Michel Pinheiro, Daisy Jereissati Barbosa Lima, Bryan Wender Debiasi, Bruno Marques Soares, Kátia da Conceição Machado, Janaina da Costa Noronha, Domingos de Jesus Rodrigues, Adilson Paulo Sinhorin, Cláudia Pessoa, and Gerardo Magela Vieira Júnior. 2013. “Antiproliferative Activity of Rhinella Marina and Rhaebo Guttatus Venom Extracts from Southern Amazon.” Toxicon 72 (September): 43–51. https://doi.org/10.1016/j.toxicon.2013.06.009.
Kaneno, R, LM Fontanari, SA Santos, LC Di Stasi, E. Rodrigues Filho, and AF Eira. 2004. “Effects of Extracts from Brazilian Sun-Mushroom (Agaricus Blazei) on the NK Activity and Lymphoproliferative Responsiveness of Ehrlich Tumor-Bearing Mice.” Food and Chemical Toxicology 42 (6): 909–16. https://doi.org/10.1016/j.fct.2004.01.014.
Martins, MC Gameiro, L Castoldi, GG Romagnoli, FC Lopes, AVFS Pinto, W Loyola, and R Kaneno. 2008. “Polysaccharide-Rich Fraction of Agaricus Brasiliensis Enhances the Candidacidal Activity of Murine Macrophages.” Memorias Do Instituto Oswaldo Cruz 103 (3): 244–50. https://doi.org/10.1590/S0074-02762008005000011.
Martins, MM Marques, SK Bussadori, RA Mesquita-Ferrari, VCS Pavesi, NS Wadt, and KP Fernandes. 2009. “Citotoxicidade in Vitro de Extratos de Arnica Brasileira (Solidago Microglossa) e Arnica Paulista (Porophyllum Ruderale).” ConScientiae Saúde 8 (1): 99–104. https://doi.org/10.5585/conssaude.v8i1.1457.
Máximo, Patrícia, and Ana Lourenço. 1998. “A Pterocarpan from Ulex Parviflorus.” Phytochemistry 48 (2): 359–62. https://doi.org/10.1016/S0031-9422(97)01090-X.
Militão, GC, INF Dantas, C Pessoa, MJC Falcão, ER Silveira, MAS Lima, R Curi, T Lima, MO Moraes, and LV Costa-Lotufo. 2006. “Induction of Apoptosis by Pterocarpans from Platymiscium Floribundum in HL-60 Human Leukemia Cells.” Life Sciences 78 (20): 2409–17. https://doi.org/10.1016/j.lfs.2005.09.044.
Militão, GC, PC Jimenez, DV Wilke, C Pessoa, MJ Falcão, MA Lima, ER Silveira, MO de Moraes, and LV Costa-Lotufo. 2005. “Antimitotic Properties of Pterocarpans Isolated from Platymiscium Floribundum on Sea Urchin Eggs.” Planta Medica 71 (7): 683–85. https://doi.org/10.1055/s-2005-871277.
Militão, GC, SM Pinheiro, INF Dantas, C Pessoa, MO de Moraes, LV Costa-Lotufo, MAS Lima, and ER Silveira. 2007. “Bioassay-Guided Fractionation of Pterocarpans from Roots of Harpalyce Brasiliana Benth.” Bioorganic & Medicinal Chemistry 15 (21): 6687–91. https://doi.org/10.1016/j.bmc.2007.08.011.
Moura, Valéria Mourão de, and Rosa Helena Veras Mourão. 2012. “Aspectos Do Ofidismo No Brasil e Plantas Medicinais Utilizadas Como Complemento à Soroterapia.” Scientia Amazonia, v. 1, N 3: 17–26. http://www.scientia.ufam.edu.br.
Moura, Valéria Mourão de, Rosa Helena Veras Mourão, and Maria Cristina Dos-Santos. 2015. “Acidentes Ofídicos Na Região Norte Do Brasil e o Uso de Espécies Vegetais Como Tratamento Alternativo e Complementar à Soroterapia.” Scientia Amazonia, no. 1: 73–84. https://doi.org/10.19178/Sci.Amazon.v4i1.73-84.
Nakagawa, Masashi, Koji Nakanishi, Laszlo L. Darko, and James A. Vick. 1982. “Structures of Cabenegrins A-I and A-II, Potent Anti-Snake Venoms.” Tetrahedron Letters 23 (38): 3855–58. https://doi.org/10.1016/S0040-4039(00)87726-6.
Oliveira, Ligianne P., Renata C. Pinheiro, Marcelo S. Vieira, José Realino Paula, Maria Teresa F. Bara, and Marize C. Valadares. 2010. “Atividade Citotóxica e Antiangiogênica de Punica Granatum L., Punicaceae.” Brazilian Journal of Pharmacognosy 20 (2): 201–7. https://doi.org/10.1590/S0102-695X2010000200011.
Pierini, S.V., D.A. Warrell, A. De Paulo, and R.D.G. Theakston. 1996. “High Incidence of Bites and Stings by Snakes and Other Animals among Rubber Tappers and Amazonian Indians of the Juruá Valley, Acre State, Brazil.” Toxicon 34 (2): 225–36. https://doi.org/10.1016/0041-0101(95)00125-5.
Pithayanukul, Pimolpan, Pakatip Ruenraroengsak, Rapepol Bavovada, Narumol Pakmanee, Rutt Suttisri, and Suwipa Saen-oon. 2005. “Inhibition of Naja Kaouthia Venom Activities by Plant Polyphenols.” Journal of Ethnopharmacology 97 (3): 527–33. https://doi.org/10.1016/j.jep.2004.12.013.
Reichert, Alana Meira, Carla Brugin Marek, Ana Maria Itinose, Renata Prestes Antonangelo, Cristiane Weirich Lenzi, Rodrigo Suzuki, and Paulino Filho Yassuda. 2014. “Biochemical Alterations Induced by Phytotherapic Tincture with Antiophidic Activity in Male Wistar Rats.” African Journal of Pharmacy and Pharmacology 8 (28): 737–46. https://doi.org/10.5897/AJPP2013.3874.
Rogero, Sizue Ota, Ademar Benévolo Lugão, Tamiko Ichikawa Ikeda, and Áurea Silveira Cruz. 2003. “Teste in Vitro de Citotoxicidade: Estudo Comparativo Entre Duas Metodologias.” Materials Research 6 (3): 317–20. https://doi.org/10.1590/s1516-14392003000300003.
Silva, AJ da, AL Coelho, AB Simas, RA Moraes, DA Pinheiro, FF Fernandes, EZ Arruda, PR Costa, and PA Melo. 2004. “Synthesis and Pharmacological Evaluation of Prenylated and Benzylated Pterocarpans against Snake Venom.” Bioorganic & Medicinal Chemistry Letters 14 (2): 431–35. https://doi.org/10.1016/j.bmcl.2003.10.044.
Silva, Fernanda Coleraus, Juliete Gomes de Lara de Souza, Alana Meira Reichert, Renata Prestes Antonangelo, Rodrigo Suzuki, Ana Maria Itinose, and Carla Brugin Marek. 2015. “Influence of the Alcohol Present in a Phytotherapic Tincture on Male Rat Lipid Profiles and Renal Function.” Evidence-Based Complementary and Alternative Medicine 2015: 1–11. https://doi.org/10.1155/2015/762373.
Silva, Graça Lúcia Da, Maria Iracema Lacerda Machado, Francisco José de Abreu Matos, and Raimundo Braz-Filho. 1999. “A New Isoflavone Isolated from Harpalyce Brasiliana.” Journal of the Brazilian Chemical Society 10 (6): 438–42. https://doi.org/10.1590/S0103-50531999000600003.
Silva, Graça Lúcia Da, Francisco José de Abreu Matos, and Edilberto Rocha Silveira. 1997. “4′-Dehydroxycabenegrin A-I from Roots of Harpalyce Brasiliana.” Phytochemistry 46 (6): 1059–62. https://doi.org/10.1016/S0031-9422(97)00338-5.
Sultana, Sabira, Hafiz Muhammad Asif, Hafiz Muhammad Irfan Nazar, Naveed Akhtar, Jalil Ur. Rehman, and Riaz Ur. Rehman. 2014. “Medicinal Plants Combating Against Cancer - a Green Anticancer Approach.” Asian Pacific Journal of Cancer Prevention 15 (11): 4385–94. https://doi.org/10.7314/APJCP.2014.15.11.4385.
Vieira, Nashira Campos, Laila Salmen Espíndola, Jaime Martins Santana, Maria Leopoldina Veras, Otília Deusdênia Loiola Pessoa, Sávio Moita Pinheiro, Renata Mendonça de Araújo, Mary Anne Sousa Lima, and Edilberto Rocha Silveira. 2008. “Trypanocidal Activity of a New Pterocarpan and Other Secondary Metabolites of Plants from Northeastern Brazil Flora.” Bioorganic & Medicinal Chemistry 16 (4): 1676–82. https://doi.org/10.1016/j.bmc.2007.11.027.
Ximenes, RM, RS Alves, TP Pereira, RM Araújo, ER Silveira, MM Rabello, MZ Hernandes, VC Soares, D Bristot, and CL Pires. 2012. “Harpalycin 2 Inhibits the Enzymatic and Platelet Aggregation Activities of PrTX-III, a D49 Phospholipase A2 from Bothrops Pirajai Venom.” BMC Complementary and Alternative Medicine 12 (1): 1161. https://doi.org/10.1186/1472-6882-12-139.
Ximenes, RM, MM Rabello, RM Araújo, ER Silveira, FHR Fagundes, EBS Diz-Filho, SC Buzzo, et al. 2012. “Inhibition of Neurotoxic Secretory Phospholipases A 2 Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce Brasiliana Benth.” Evidence-Based Complementary and Alternative Medicine, 1–9. https://doi.org/10.1155/2012/987517.
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2020-03-05
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CASTOLDI, Lindsey; ALBIERO, Lucinéia Reuse; NERY, Eduardo Figueredo; KELLY, Taiany Oliveira; DALAZEN, Jeniffer Charlene Silva; MASOCHINI, Rosângela Guerino. Evaluation of in vitro Cytotoxic Effects of Especifico Pessoa Phytotherapic Tincture on Ehrlich Tumor Cells and Mice Spleen Cells. Fronteira: Journal of Social, Technological and Environmental Science, [S. l.], v. 9, n. 1, p. 458–472, 2020. DOI: 10.21664/2238-8869.2020v9i1.p458-472. Disponível em: https://periodicos.unievangelica.edu.br/index.php/fronteiras/article/view/2975. Acesso em: 24 nov. 2024.
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