Morphoanatomical Study and Antimicrobial Activity of Essential Oil, Crude Extract and Fractions from Miconia cuspidata Naudin Aerial Parts
DOI:
https://doi.org/10.21664/2238-8869.2023v12i3.p224-238Keywords:
vegetal anatomy, crude extract, antimicrobial, CandidaAbstract
Miconia cuspidate Naudin, widely known as "pixirica"or "sabiazeira-da-folha-fina", is a tree that belongs to Melastomataceae family. The aim of this work was making the morphoanatomical study, and evaluate the antimicrobial activity of essential oil, crude ethanol extract and hexane, dichloromethane, ethyl acetate, and aqueous fractions from M. cuspidata leaves. The morphological and anatomical studies were accomplished according to conventional techniques of vegetal anatomy. The essential oil obtained by hydrodistillation in a Clevenger apparatus. Antimicrobial activity was assessed by determining the Minimal Inhibitory Concentration (MIC) using the broth serial microdilution test. The study showed M. cuspidate is a tree about 5m high, has cylindrical stem, grayish bark, simple, opposite and short petiole leaves, white flowers, bacaceous, juicy and purplish fruit. And small, numerous and light brownish seeds. The leaf is hypostomatic with diacytic and anomocytic stomata. The mesophyll is dorsiventral, presenting palisade parenchyma with two layers of cells and spongy parenchyma with 4 to 5 layers of cells. Was observed presence of simple glandular trichomes containing oil inside. The main vein is concave-convex with cortical parenchyma presenting 7 layers of cells, druse and sclerified cells.The petiole has a convex-slightly concave outline. The young twig, in cross-section, has an oval shape with grooves in the central region. The crude ethanol extract and dichloromethane, ethyl acetate, and aqueous fractions from aerial parts showed strong antimicrobial activity (CIM = 4-16 μg/Ml) against Candida glabrata and Candida krusei.
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