Micropropagação de Espécies Lenhosas Nativas: Análise do Conhecimento Científico e Perspectivas para a Conservação

Autores

DOI:

https://doi.org/10.21664/2238-8869.2025v14i1.p267-298

Palavras-chave:

biomas brasileiros, cienciometria, biotecnologia

Resumo

A micropropagação é uma técnica promissora para a multiplicação em larga escala de plantas, contribuindo para a conservação genética, restauração de ecossistemas e desenvolvimento econômico sustentável, especialmente de espécies medicinais e madeireiras. Este estudo analisou 645 publicações indexadas nas bases Web of Science e Scopus entre 2013 e 2023. Após excluir duplicatas, 612 publicações foram selecionadas, das quais 31 atenderam aos critérios, focando espécies lenhosas nos domínios fitogeográficos brasileiros. A técnica foi mais aplicada no Cerrado (25%) e na Mata Atlântica (22%), enquanto a Amazônia representou 14%. Três estudos citaram o bioma no título, destacando a importância de contextualizar espécies em seus habitats ecológicos. O meio de cultura WPM foi usado em 80% dos estudos, seguido pelo MS em 67%. Alternativas como JADS, DKW e QL foram menos frequentes, mas promissoras. Entre os reguladores de crescimento, o Benzilaminopurina (BAP) foi destaque em 30% das pesquisas, seguido pelo Ácido Indolbutírico (AIB) em 20%, especialmente para enraizamento. Suplementos como carvão ativado, polivinilpirrolidona (PVP), caseína hidrolisada e Plant Preservative Mixture (PPM) foram usados para otimizar o crescimento. Explantes nodais foram predominantes (38%), seguidos por sementes (18%). Os resultados destacam a importância de ampliar pesquisas voltadas para a conservação ex situ e o uso sustentável da biodiversidade brasileira, considerando a necessidade de desenvolver e adaptar protocolos de cultura que atendam às demandas específicas de cada espécie, promovendo avanços significativos na preservação de recursos genéticos e no aproveitamento sustentável das plantas. Além disso, ao analisar a produção científica, torna-se possível direcionar esforços de conservação para biomas específicos sob maior ameaça.

Referências

A., S., Sileshi, G., du Toit, E. S., & K., F. 2012 Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures. Em Fungicides for Plant and Animal Diseases. InTech. https://doi.org/10.5772/27662

Abhilash, P. C. 2021 Restoring the Unrestored: Strategies for Restoring Global Land during the UN Decade on Ecosystem Restoration (UN-DER Land, 10(2), 201. https://doi.org/10.3390/land10020201

Ahuja, M. R. Ed. 2013 Micropropagation of woody plants. Vol. 41 Springer Science & Business Media.

Aleixo, A., Luisa Albernaz, A., Eduardo Viveiros Grelle, C., Moncassim Vale, M., & Fernando Rangel, T. 2010 Mudanças Climáticas e a Biodiversidade dos Biomas Brasileiros: Passado, Presente e Futuro. Natureza & Conservação, 08(02), 194–196. https://doi.org/10.4322/natcon.00802016

Ali, H. M., Khan, T., Khan, M. A., & Ullah, N. 2022 The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro. Biotechnology and Applied Biochemistry, 69(6), 2624–2640. https://doi.org/10.1002/bab.2311

Amirova, A., Dossymbetova, S., Rysbayeva, Y., Usenbekov, B., Tolegen, A., & Ydyrys, A. 2022 Multiple Plant Regeneration from Embryogenic Calli of Paulownia tomentosa (Thunb.) Steud. Plants, 11(8), 1020. https://doi.org/10.3390/plants11081020

Artunduaga, I. R., Taliaferro, C. M., & Johnson, B. B. 1989 Induction and growth of callus from immature inflorescences of “Zebra” bermudagrass as affected by casein hydrolysate and 2,4-D concentration. In Vitro Cellular & Developmental Biology, 25(8), 753–756. https://doi.org/10.1007/BF02623729

Araujo, M. C. R., Vendrame, W. A., Chagas, E. A., Ribeiro, M. I. G., & Vilaca, R. 2019 Preliminary Studies on In Vitro Propagation of Camu-Camu ( Myrciaria dubia ), an Important Medicinal Plant. Proceedings of the Florida State Horticultural Society, 128, 52–54. https://www.cabdirect.org/cabdirect/abstract/20193451356

Aydin, Y., Ipekci, Z., Talas-Oğraş, T., Zehir, A., Bajrovic, K., & Gozukirmizi, N. 2004 High Frequency Somatic Embryogenesis in Cotton. Biologia plantarum, 48(4), 491–495. https://doi.org/10.1023/B:BIOP.0000047142.07987.e1

Barbosa, A. S. 2016 Implicações éticas do efeito Mateus na ciência. Mediações Revista De Ciências Sociais, 21(1), 286. https://doi.org/10.5433/2176-6665.2016v21n1p286

Bairu, M. W., Stirk, W. A., Dolezal, K., & Van Staden, J. 2007 Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell, Tissue and Organ Culture, 90(1), 15–23. https://doi.org/10.1007/s11240-007-9233-4

Ballesteros, D., Fanega-Sleziak, N., & Davies, R. M. 2021 Cryopreservation of Seeds and Seed Embryos in Orthodox-, Intermediate-, and Recalcitrant-Seeded Species (p. 663–682 https://doi.org/10.1007/978-1-0716-0783-1_36

Bao, J., O’Donohue, B., Sommerville, K. D., Mitter, N., O’Brien, C., & Hayward, A. 2024 Tissue Culture Innovations for Propagation and Conservation of Myrteae—A Globally Important Myrtaceae Tribe. Plants, 13(16), 2244. https://doi.org/10.3390/plants13162244

Barata, R. B. 2019 Mudanças necessárias na avaliação da pós-graduação brasileira. Interface - Comunicação, Saúde, Educação, 23. https://doi.org/10.1590/interface.180635

Bazerman, C. 1985 Physicists Reading Physics. Written Communication, 2(1), 3–23. https://doi.org/10.1177/0741088385002001001

Bhatia, S., & Sharma, K. 2015 Chapter 14—Plant Tissue Culture-Based Industries. Modern Applications of Plant Biotechnology in Pharmaceutical Sciences; Bhatia, S., Sharma, K., Dahiya, R., Bera, T., Eds, 405-417.

Begna, T. 2020 The Role of Genotype by Environmental Interaction in Plant Breeding. Journal of Natural Sciences Research, 209–215. https://doi.org/10.7176/JNSR/11-20-02

Bazerman, C., (1988 Shaping written knowledge: The genre and activity of the experimental article in science. The University of Wisconsin Press google schola, 2, .345-348.

Bejaoui, M., Galai, H., Touati, F., & Kouass, S. 2023 Multifunctional Roles of PVP as a Versatile Biomaterial in Solid State. Em Dosage Forms - Innovation and Future Perspectives. IntechOpen. https://doi.org/10.5772/intechopen.99431

Bister-Miel, F., Guignard, J. L., Bury, M., & Agier, C. 1985 Glutamine as an active component of casein hydrolysate: Its balancing effect on plant cells cultured in phosphorus deficient medium. Plant Cell Reports, 4(3), 161–163. https://doi.org/10.1007/BF00571307

Borges, Mário Neto. Perspectivas de Financiamento da Pesquisa Científica no Brasil: O século XXI, conhecido como o século do conhecimento, tem apresentado avanços científicos e tecnológicos muito rápidos e impressionantes. Inovação & Desenvolvimento: A Revista da FACEPE, v. 1, n. 7, p. 20-28, 2021.

Bornmann, L., & Leydesdorff, L. 2014 Scientometrics in a changing research landscape. EMBO reports, 15(12), 1228–1232. https://doi.org/10.15252/embr.201439608

Borokini, T. I., Okere, A. U., Olusesan Giwa, A., Daramola, B. O., & Odofin, W. T. 2010 Biodiversity and conservation of plant genetic resources in Field Genebank of the National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria. Em International Journal of Biodiversity and Conservation (Vol. 2, Número 3 http://www.academicjournals.org/ijbc

Bradley, P. M., & Cheney, D. P. 1990 Some effects of plant growth regulators on tissue cultures of the marine red alga Agardhiella subulata (Gigartinales, Rhodophyta Hydrobiologia, 204–205(1), 353–360. https://doi.org/10.1007/BF00040256

Brandão, D. O., Barata, L. E. S., & Nobre, C. A. 2022 The Effects of Environmental Changes on Plant Species and Forest Dependent Communities in the Amazon Region. Forests, 13(3), 466. https://doi.org/10.3390/f13030466

Burley, J. 1989 Applications of Biotechnology in Forestry and Rural Development. Em Applications of Biotechnology in Forestry and Horticulture (p. 9–20 Springer US. https://doi.org/10.1007/978-1-4684-1321-2_2

Burns, C. S., & Islam, Md. A. 2024 A citation analysis examining geographical specificity in article titles. Scientometrics, 129(7), 4317–4328. https://doi.org/10.1007/s11192-024-05075-3

Buxton, A. B., & Meadows, A. J. 1977 The variation in the information content of titles of research papers with time and discipline. Journal of Documentation, 33(1), 46–52. https://doi.org/10.1108/eb026633

Cai, X., Wei, H., Liu, C., Ren, X., Thi, L. T., & Jeong, B. R. 2020 Synergistic Effect of NaCl Pretreatment and PVP on Browning Suppression and Callus Induction from Petal Explants of Paeonia Lactiflora Pall. ‘Festival Maxima’. Plants, 9(3), 346. https://doi.org/10.3390/plants9030346

Calò, L. N. 2022 Métricas de impacto y evaluación de la ciencia. Revista Peruana de Medicina Experimental y Salud Pública, 39(2), 236–240. https://doi.org/10.17843/rpmesp.2022.392.11171

Cardoso, J. C., & Inthurn, A. C. P. 2018 Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2 Ornamental Horticulture, 24(3), 218–224. https://doi.org/10.14295/oh.v24i3.1222

Cardoso, J. C., Sheng Gerald, L. T., & Teixeira da Silva, J. A. 2018 Micropropagation in the Twenty-First Century (p. 17–46 https://doi.org/10.1007/978-1-4939-8594-4_2

Cassells, A. C., & Curry, R. F. 2001 Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell, Tissue and Organ Culture, 64(2/3), 145–157. https://doi.org/10.1023/A:1010692104861

Catanzaro, M., Miranda, G., Palmer, L., & Bajak, A. 2014 South American science: Big players. Nature, 510(7504), 204–206. https://doi.org/10.1038/510204a

Cazalis, V., Di Marco, M., Butchart, S. H. M., Akçakaya, H. R., González-Suárez, M., Meyer, C., Clausnitzer, V., Böhm, M., Zizka, A., Cardoso, P., Schipper, A. M., Bachman, S. P., Young, B. E., Hoffmann, M., Benítez-López, A., Lucas, P. M., Pettorelli, N., Patoine, G., Pacifici, M., … Santini, L. 2022 Bridging the research-implementation gap in IUCN Red List assessments. Trends in Ecology & Evolution, 37(4), 359–370. https://doi.org/10.1016/j.tree.2021.12.002

Chokheli, V. A., Dmitriev, P. A., Rajput, V. D., Bakulin, S. D., Azarov, A. S., Varduni, T. V., Stepanenko, V. V., Tarigholizadeh, S., Singh, R. K., Verma, K. K., & Minkina, T. M. 2020 Recent Development in Micropropagation Techniques for Rare Plant Species. Plants, 9(12), 1733. https://doi.org/10.3390/plants9121733

Collar, N. J. 1996 Species concepts and conservation: a response to Hazevoet. Bird Conservation International, 6(2), 197–200. https://doi.org/10.1017/S0959270900003075

Conceição, B. C. da, Silva, T. A. da, Pantoja, L. V. P. da S., Luz, D. A. da, Cardoso, E. K. S., Reis, L. D. da S., Raiol-da-Silva, M. C., Kussler, M. S., Maia, C. S. F., & Fontes-Júnior, E. A. 2023 Amazonian Plants: A Global Bibliometric Approach to Petiveria alliacea L. Pharmacological and Toxicological Properties. Plants, 12(18), 3343. https://doi.org/10.3390/plants12183343

Corrales-Reyes, I. E., Fornaris-Cedeño, Y., & Reyes-Pérez, J. J. 2018 Análisis bibliométrico de la revista investigación en educación médica. Período 2012-2016. Investigación en Educación Médica, 7(25), 18–26. https://doi.org/10.1016/j.riem.2017.02.003

Corredoira, E., Martínez, M. T., Sanjosé, M. C., & Ballester, A. 2017 Conservation of Hardwood Forest Species (p. 421–453 https://doi.org/10.1007/978-3-319-66426-2_14

Cuckston, T. 2018 Making extinction calculable. Accounting, Auditing & Accountability Journal, 31(3), 849–874. https://doi.org/10.1108/AAAJ-10-2015-2264

Cunsolo, A., & Ellis, N. R. 2018 Ecological grief as a mental health response to climate change-related loss. Nature Climate Change, 8(4), 275–281. https://doi.org/10.1038/s41558-018-0092-2

Custódio, L., Charles, G., Magné, C., Barba-Espín, G., Piqueras, A., Hernández, J. A., Ben Hamed, K., Castañeda-Loaiza, V., Fernandes, E., & Rodrigues, M. J. 2022 Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review. Plants, 12(1), 126. https://doi.org/10.3390/plants12010126

Delgado-Paredes, G. E., Vásquez-Díaz, C., Esquerre-Ibañez, B., Bazán-Sernaqué, P., & Rojas-Idrogo, C. 2021 In vitro tissue culture in plants propagation and germplasm conservation of economically important species in Peru. Scientia agropecuaria, 12(3), 337–349. https://doi.org/10.17268/sci.agropecu.2021.037

De Jesus Silva, H. F., Asmar, S. A., De Oliveira, R. C., De Melo, B., Luz, J. M. Q., & Pasqual, M. 2016 In vitro establishment and early development of barueiro (Dipteryx alata Vogel Semina Ciências Agrárias, 37(4), 1779. https://doi.org/10.5433/1679-0359.2016v37n4p1779

dos Santos, M. A. C., do Rêgo, M. M., de Queiróz, M. A., Caproni, D. T. R., Dietrich, O. H. S., Santos, A. F., Rocha, D. I., Batista, D. S., & Otoni, W. C. 2020 In vitro growth performance of Psidium guajava and P. guineense plantlets as affected by culture medium formulations. Vegetos, 33(3), 435–445. https://doi.org/10.1007/s42535-020-00125-6

Falkiner, F. R. 1997 Antibiotics in Plant Tissue Culture and Micropropagation — What are We Aiming at? (p. 155–160 https://doi.org/10.1007/978-94-015-8951-2_18

FAO/IAEA. 2002 Low-Cost Options for Tissue Culture Technology in Developing Countries. In Proceedings of the Technical Meeting Organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria, 26–30 August 2002; Available from: https://www-pub.iaea.org/mtcd/publications/pdf/te_1384_web.pdf acesso: 14 de Junho de 2024.

Faria, L. I. L. et al. 2011 Análise da produção científica a partir de publicações em periódicos especializados. In: fun-dação de amparo à pesquisa do estado de são paulo. Indicadores de ciência, tecnologia e inovação em São Paulo 2010. São Paulo: FAPESP, 2011.

Fizikova, A., Subcheva, E., Kozlov, N., Tvorogova, V., Samarina, L., Lutova, L., & Khlestkina, E. 2024 Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects. Plants, 13(5), 675. https://doi.org/10.3390/plants13050675

Fonseca, C. R., & Venticinque, E. M. 2018 Biodiversity conservation gaps in Brazil: A role for systematic conservation planning. Perspectives in Ecology and Conservation, 16(2), 61–67. https://doi.org/10.1016/j.pecon.2018.03.001

Fraser, N., Brierley, L., Dey, G., Polka, J. K., Pálfy, M., Nanni, F., & Coates, J. A. 2021 The evolving role of preprints in the dissemination of COVID-19 research and their impact on the science communication landscape. PLOS Biology, 19(4), e3000959. https://doi.org/10.1371/journal.pbio.3000959

Fridborg, G., Pedersén, M., Landström, L., & Eriksson, T. 1978 The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis. Physiologia Plantarum, 43(2), 104–106. https://doi.org/10.1111/j.1399-3054.1978.tb01575.x

Gaidamashvili, M., & Benelli, C. 2021 Threatened Woody Plants of Georgia and Micropropagation as a Tool for In Vitro Conservation. Agronomy, 11(6), 1082. https://doi.org/10.3390/agronomy11061082

Garcia, C., Furtado de Almeida, A.-A., Costa, M., Britto, D., Valle, R., Royaert, S., & Marelli, J.-P. 2019 Abnormalities in somatic embryogenesis caused by 2,4-D: an overview. Plant Cell, Tissue and Organ Culture (PCTOC), 137(2), 193–212. https://doi.org/10.1007/s11240-019-01569-8

García-Luis, A., Molina, R. V., Varona, V., Castelló, S., & Guardiola, J. L. 2006 The influence of explant orientation and contact with the medium on the pathway of shoot regeneration in vitro in epicotyl cuttings of Troyer citrange. Plant Cell, Tissue and Organ Culture, 85(2), 137–144. https://doi.org/10.1007/s11240-005-9060-4

George, E. F., Hall, M. A., & Klerk, G.-J. De. 2008 Micropropagation: Uses and Methods. Em Plant Propagation by Tissue Culture (p. 29–64 Springer Netherlands. https://doi.org/10.1007/978-1-4020-5005-3_2

Glänzel, W., & Heeffer, S. 2014 Cross-national preferences and similarities in downloads

and citations of scientific articles: A pilot study. In Proceedings of the 19th International

Conference on Science and Technology Indicators (STI2014) (pp. 207–215

Netherlands: Leiden University.

Gouveia, S. F., Souza‐Alves, J. P., Rattis, L., Dobrovolski, R., Jerusalinsky, L., Beltrão‐Mendes, R., & Ferrari, S. F. 2016 Climate and land use changes will degrade the configuration of the landscape for titi monkeys in eastern Brazil. Global Change Biology, 22(6), 2003–2012. https://doi.org/10.1111/gcb.13162

Grácio, M. C. C., & Oliveira, E. F. T. de. 2014 Indicadores cientométricos normalizados: um estudo na produção científica brasileira internacional (1996 a 2011 Perspectivas em Ciência da Informação, 19(3), 118–133. https://doi.org/10.1590/1981-5344/1898

Grimaldi, F., & Assumpção Bastos, F. E. 2023 Control of in vitro contamination during the establishment of Pyrus communis explants using Plant Preservative MixtureTM. Plant Cell Culture & Micropropagation. https://doi.org/10.46526/pccm.2023.v19.185

Goode, W. J., & Hatt, P. F. 1969 Alguns problemas na análise qualitativa e na análise de caso. In W. J. Goode & P. F. Hatt (Orgs.), Métodos em pesquisa social (pp. 398-433 São Paulo: Companhia Editora Nacional.

Guimarães, P. de O., Santos, A. M. dos, Silva, C. F. A. da, Rudke, A. P., Nunes, F. G., Lisboa, G. dos S., & Fernandes, M. de S. 2023 Spatial analysis of deforestation factors in the Atlantic Forest Biome/Brazil. Revista Geografias, 19(1), 1–19. https://doi.org/10.35699/2237-549X.2023.42662

Gurel, E., & Wren, M. 1995 development from leaf explants of sugar beet ( L Rhizogenesis and the effect of sequential exposure to auxin and cytokinin. Annals of Botany, 75(1), 31–38. https://doi.org/10.1016/S0305-7364(05)80006-X

Hashim, S. N., Ghazali, S. Z., Sidik, N. J., Chia-Chay, T., & Saleh, A. 2021 Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture. E3S Web of Conferences, 306, 01004. https://doi.org/10.1051/e3sconf/202130601004

Hasnain, A., Naqvi, S. A. H., Ayesha, S. I., Khalid, F., Ellahi, M., Iqbal, S., Hassan, M. Z., Abbas, A., Adamski, R., Markowska, D., Baazeem, A., Mustafa, G., Moustafa, M., Hasan, M. E., & Abdelhamid, M. M. A. 2022 Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.1009395

Henkhaus, N., Bartlett, M., Gang, D., Grumet, R., Jordon‐Thaden, I., Lorence, A., Lyons, E., Miller, S., Murray, S., Nelson, A., Specht, C., Tyler, B., Wentworth, T., Ackerly, D., Baltensperger, D., Benfey, P., Birchler, J., Chellamma, S., Crowder, R., Stern, D. 2020 Plant science decadal vision 2020–2030: Reimagining the potential of plants for a healthy and sustainable future. Plant Direct, 4(8 https://doi.org/10.1002/pld3.252

Hussain, Altaf et al. 2012 Plant tissue culture: current status and opportunities. Recent advances in plant in vitro culture. 6(10 1-28.

Ho, W. J., Huang, Y. K., Huang, W. W., Huang, Y. C., & Chung, J. P. 2022 Effective in vitro culture using dormant bud of nodal sections from a mature Acacia tree. In Vitro Cellular and Developmental Biology - Plant, 58(3), 437–446. https://doi.org/10.1007/s11627-021-10235-8

Huang, Y.-J., Cheng, S., Yang, F.-Q., & Chen, C. 2022 Analysis and Visualization of Research on Resilient Cities and Communities Based on VOSviewer. International Journal of Environmental Research and Public Health, 19(12), 7068. https://doi.org/10.3390/ijerph19127068

Hussain, A., Ahmed, I., Nazir, H., & Ullah, I. 2012 Plant Tissue Culture: Current Status and Opportunities. Em Recent Advances in Plant in vitro Culture. InTech. https://doi.org/10.5772/50568

IUCN. 2024, “Overview of The IUCN Red List, Available from: www.iucnredlist.org/about/overview. Acesso em: 3 out. 2024.

Ikeuchi, M., Sugimoto, K., & Iwase, A. 2013 Plant Callus: Mechanisms of Induction and Repression. The Plant Cell, 25(9), 3159–3173. https://doi.org/10.1105/tpc.113.116053

Jain, S. M., & Häggman, H. Eds. 2007 Protocols for micropropagation of woody trees and fruits. Springer Science & Business Media.

Jesus, R. da S., Oliveira, L. S., Silva, L. M., & Carvalho, L. R. 2024 RESGATE E CONSERVAÇÃO IN VITRO DE ESPÉCIES FLORESTAIS NATIVAS AMEAÇADAS. Em Tópicos Especiais em Engenharia Florestal - Volume 2 (p. 182–201 Editora Científica Digital. https://doi.org/10.37885/240717276

Jiang, F. K., & Hyland, K. 2023 Titles in research articles: Changes across time and discipline. Learned Publishing, 36(2), 239–248. https://doi.org/10.1002/leap.1498

Johansson, L. 1983 Effects of activated charcoal in anther cultures. Physiologia Plantarum, 59(3), 397–403. https://doi.org/10.1111/j.1399-3054.1983.tb04221.x

Keith, D. A., Rodríguez, J. P., Brooks, T. M., Burgman, M. A., Barrow, E. G., Bland, L., Comer, P. J., Franklin, J., Link, J., McCarthy, M. A., Miller, R. M., Murray, N. J., Nel, J., Nicholson, E., Oliveira‐Miranda, M. A., Regan, T. J., Rodríguez‐Clark, K. M., Rouget, M., & Spalding, M. D. 2015 The IUCN Red List of Ecosystems: Motivations, Challenges, and Applications. Conservation Letters, 8(3), 214–226. https://doi.org/10.1111/conl.12167

Khaleghi, M. R. 2017 The influence of deforestation and anthropogenic activities on runoff generation. Journal of Forest Science, 63(6), 245–253. https://doi.org/10.17221/130/2016-JFS

Krob, A., Overbeck, G. E., Mähler Jr., J. K. F., Urruth, L., Malabarba, L. R., Chomenko, L., & Azevedo, M. A. 2021 Contribution of southern Brazil to the climate and biodiversity conservation agenda. Bio Diverso, 1(1 Recuperado de https://seer.ufrgs.br/index.php/biodiverso/article/view/120228

Koleva, P., Petrova, N., Krumova, S., Velikova, V., Aneva, I., Evstatieva, L., Wolfram, E., & Danova, K. 2016 Effect of activated charcoal on the developmental patterns, polyphenolics productivity and photosynthetic activity of Sideritis scardica in vitro. Planta Medica, 81(S 01), S1–S381. https://doi.org/10.1055/s-0036-1596817

Kuczak, M., & Kurczyńska, E. 2020 Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced. International Journal of Molecular Sciences, 21(21), 8126. https://doi.org/10.3390/ijms21218126

Kulak, V., Longboat, S., Brunet, N. D., Shukla, M., & Saxena, P. 2022 In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity. Plants, 11(4), 503. https://doi.org/10.3390/plants11040503

Kumar, T., . R., Sethiya, R., . P., Thakur, S., & . S. 2024 A comprehensive review of Plant Growth Regulators (PGRs) and their impact on flowering and ornamental crops with insights into effective application methods. International Journal of Advanced Biochemistry Research, 8(2S), 254–267. https://doi.org/10.33545/26174693.2024.v8.i2Sd.553

Kyte, L.; Kleyn, J. O. H. N. 1996 Plants from test tubes: an introduction to micropropagation.

Lavery, T. H., Morgain, R., Fitzsimons, J. A., Fluin, J., Macgregor, N. A., Robinson, N. M., Scheele, B. C., Selwood, K. E., Spindler, R., Vuong, H., West, S., Wintle, B. A., & Lindenmayer, D. B. 2021 Impact Indicators for Biodiversity Conservation Research: Measuring Influence within and beyond Academia. BioScience, 71(4), 383–395. https://doi.org/10.1093/biosci/biaa159

Leeds University. 2023 Amazon: Challenges of science in the rainforest. University of Leeds. Available from: https://environment.leeds.ac.uk/geography/news/article/5639/amazon-challenges-of-science-in-the-rainforest. Acesso em: 22 abril 2024.

Leta, J., Glänzel, W., & Thijs, B. 2006 Science in Brazil. Part 2: Sectoral and institutional research profiles. Scientometrics, 67(1), 87–105. https://doi.org/10.1007/s11192-006-0051-y

Leta, J., Thijs, B., & Glänzel, W. 2013 A macro-level study of science in Brazil: seven years later. Encontros Bibli: revista eletrônica de biblioteconomia e ciência da informação, 18(36), 51–66. https://doi.org/10.5007/1518-2924.2013v18n36p51

Letham, D. S. 1967 Regulators of cell division in plant tissues. Planta, 74(3), 228–242. https://doi.org/10.1007/BF00384844

Liu, X., Zhu, K., & Xiao, J. 2023 Recent advances in understanding of the epigenetic regulation of plant regeneration. aBIOTECH, 4(1), 31–46. https://doi.org/10.1007/s42994-022-00093-2

Long, Y., Yang, Y., Pan, G., & Shen, Y. 2022 New Insights Into Tissue Culture Plant-Regeneration Mechanisms. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.926752

Lloyd., G., B., McCown. 1980 Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. 30, 421-427.

Mace, G. M., Collar, N. J., Gaston, K. J., Hilton‐Taylor, C., Akçakaya, H. R., Leader‐Williams, N., Milner‐Gulland, E. J., & Stuart, S. N. 2008 Quantification of Extinction Risk: IUCN’s System for Classifying Threatened Species. Conservation Biology, 22(6), 1424–1442. https://doi.org/10.1111/j.1523-1739.2008.01044.x

Mace, G. M., Norris, K., & Fitter, A. H. 2012 Biodiversity and ecosystem services: a multilayered relationship. Trends in Ecology & Evolution, 27(1), 19–26. https://doi.org/10.1016/j.tree.2011.08.006

Mattedi, M. A., & Spiess, M. R. 2017 A avaliação da produtividade científica. História, Ciências, Saúde-Manguinhos, 24(3), 623–643. https://doi.org/10.1590/s0104-59702017000300005

Máximo, W. P. F., Barbosa, S., Martins, J. P. R., Santos, B. R., & Beijo, L. A. 2020 Multiplication and; n vitro rooting of ;Handroanthus impetiginosu (Mart. Ex DC.) Mattos. Ciência Florestal, 30(3), 658–668. https://doi.org/10.5902/1980509827012

Mazloumian, A., Helbing, D., Lozano, S., Light, R. P., & Börner, K. 2013 Global Multi-Level Analysis of the ‘Scientific Food Web’’’. Scientific Reports, 3(1), 1167. https://doi.org/10.1038/srep01167

Mcmanus, C. M., Neves, A. A. B., & Maranhão, A. Q. 2020 Brazilian Publication Profiles: Where and How Brazilian authors publish. Anais da Academia Brasileira de Ciências, 92(2 https://doi.org/10.1590/0001-3765202020200328

Mccown, B. H.; Sellmer, J. C. 1987 General media and vessels suitable for woody plant culture. Cell and tissue culture in forestry: general principles and biotechnology. Dordrecht: Springer Netherlands, 4-16.

Mehta, N., Inamdar, V., Puthillam, A., Chunekar, S., Kapoor, H., Tagat, A., & Subramanyam, D. 2023 Assessing the impact of COVID-19 on STEM (science, technology, engineering, mathematics) researchers in India. Wellcome Open Research, 7, 157. https://doi.org/10.12688/wellcomeopenres.17853.2

Merton, R. K. 1968 The Matthew Effect in Science. Science, 159(3810), 56–63. https://doi.org/10.1126/science.159.3810.56

Milojević, S., Sugimoto, C. R., Yan, E., & Ding, Y. 2011 The cognitive structure of Library and Information Science: Analysis of article title words. Journal of the American Society for Information Science and Technology, 62(10), 1933–1953. https://doi.org/10.1002/asi.21602

Miglioli, S. 2018) Influência E Limites Do Fator De Impacto Como Métrica De Avaliação Na Ciência. PontodeAcesso, 11(3), 17–33, Available from: https://periodicos.ufba.br/index.php/revistaici/article/view/17263. Acesso em: 3 out. 2024.

Mng'omba, Simon A. et al. 2012 Efficacy and utilization of fungicides and other antibiotics for aseptic plant cultures. InTech Open Access Publisher.

Mugnaini, R., Damaceno, R. J. P., Digiampietri, L. A., & Mena-Chalco, J. P. 2019 Panorama da produção científi ca do Brasil além da indexação: uma análise exploratória da comunicação em periódicos. Transinformação, 31. https://doi.org/10.1590/2318-0889201931e190033

Murthy, H. N., Joseph, K. S., Paek, K. Y., & Park, S. Y. 2023 Bioreactor systems for micropropagation of plants: present scenario and future prospects. Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1159588

Niedz, R. P., & Bausher, M. G. 2002 Control of in vitro contamination of explants from greenhouse- and field-grown trees. In Vitro Cellular & Developmental Biology - Plant, 38(5), 468–471. https://doi.org/10.1079/IVP2002316

Nassi-Caló, L. 2014 A ciência na América do Sul na Nature. Scielo em Perspectiva. Available from: https://blog.scielo.org/blog/2014/07/04/a-ciencia-na-america-do-sul-na-nature/. Acesso em 20 nov 2024.

Normah, M. N., Chin, H. F., & Reed, B. M. 2013 Conservation of Tropical Plant Species (M. N. Normah, H. F. Chin, & B. M. Reed, Orgs. Springer New York. https://doi.org/10.1007/978-1-4614-3776-5

Oliveira Junior, M. A. de, Brogio Colli, B. do A., Libório Stipp, L. C., Latado, R. R., Stefano Piedade, S. M. De, & Mourão Filho, F. de A. A. 2024 In vitro culture of Rio Grande cherry (Eugenia involucrata DC. Plant Cell, Tissue and Organ Culture (PCTOC), 157(1), 21. https://doi.org/10.1007/s11240-024-02735-3

Omary, M. B., Eswaraka, J., Kimball, S. D., Moghe, P. V., Panettieri, R. A., & Scotto, K. W. 2020 The COVID-19 pandemic and research shutdown: staying safe and productive. Journal of Clinical Investigation, 130(6), 2745–2748. https://doi.org/10.1172/JCI138646

OPRINS, Jan et al. 2004 Micropropagation: a general method for commercial bamboo production. In: World bamboo congress. p. 1-11.

Oseni, O. M., Pande, V., & Nailwal, T. K. 2018 A Review on Plant Tissue Culture, A Technique for Propagation and Conservation of Endangered Plant Species. International Journal of Current Microbiology and Applied Sciences, 7(07), 3778–3786. https://doi.org/10.20546/ijcmas.2018.707.438

Paranhos, J. T., Löbler, L., Cechin, J., Fernandes, T. S., Fao, E., & Soriani, H. H. 2017 Propagação in vitro de Casearia sylvestris</i> SWARTZ (SALICACEAE Ciência Florestal, 27(4), 1191–1199. https://doi.org/10.5902/1980509830301

Pereira, J. E. Ed. 2012 Contaminações microbianas na cultura de células, tecidos e órgãos de plantas (2ª ed. Brasília, DF: Embrapa. https://www.embrapa.br/ebook

Phillips, G. C., & Garda, M. 2019 Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology - Plant, 55(3), 242–257. https://doi.org/10.1007/s11627-019-09983-5

Pinto, A. S., Monteiro, F. K. da S., Ramos, M. B., Araújo, R. da C. C., & Lopes, S. de F. 2020 Invasive plants in the Brazilian Caatinga: a scientometric analysis with prospects for conservation. Neotropical Biology and Conservation, 15(4), 503–520. https://doi.org/10.3897/neotropical.15.e57403

Powell, W. W., & Snellman, K. 2004 The Knowledge Economy. Annual Review of Sociology, 30(1), 199–220. https://doi.org/10.1146/annurev.soc.29.010202.100037

Prakash, S., & Verma, A. K. 2022 Anthropogenic Activities And Biodiversity Threats. International Journal of Biological Innovations, 04(01), 94–103. https://doi.org/10.46505/IJBI.2022.4110

Raghuvanshi, S. S., & Srivastava, A. 1995 Plant regeneration of Mangifera indica using liquid shaker culture to reduce phenolic exudation. Plant Cell, Tissue and Organ Culture, 41(1), 83–85. https://doi.org/10.1007/BF00124092

Ranghoo-Sanmukhiya, V. M. 2021 Somaclonal Variation and Methods Used for Its Detection. Em Propagation and Genetic Manipulation of Plants (. 1–18 Springer Singapore. https://doi.org/10.1007/978-981-15-7736-9_1

Rahman SS. 2018 DKW emerges as a superior media factor in in vitro plant

regeneration. J Agri. 1(1):3-4.

Rathore, J. S., Rathore, V., Shekhawat, N. S., Singh, R. P., Liler, G., Phulwaria, M., & Dagla, H. R. 2004 Micropropagation of woody plants. In Plant biotechnology and molecular markers.195-205. Dordrecht: Springer Netherlands.

Razera, J. C. C. 2016 Contribuições da cienciometria para a área brasileira de Educação em Ciências. Ciência & Educação (Bauru), 22(3), 557–560. https://doi.org/10.1590/1516-731320160030001

Rezende, S. M. 2011 Produção científica e tecnológica no Brasil: conquistas recentes e desafios para a próxima década. Revista de Administração de Empresas, 51(2), 202–209. https://doi.org/10.1590/S0034-75902011000200007

Ribeiro, D. B., Oliveira, E. F. dos A., & Garcia, M. L. T. 2023 Retrocessos no financiamento da Ciência, Tecnologia e Inovação no Brasil: o caso do CNPq. Serviço Social & Sociedade, 146(3 https://doi.org/10.1590/0101-6628.326

Ritter, M. R., Silva, T. C. da, Araújo, E. de L., & Albuquerque, U. P. 2015 Bibliometric analysis of ethnobotanical research in Brazil (1988-2013 Acta Botanica Brasilica, 29(1), 113–119. https://doi.org/10.1590/0102-33062014abb3524

Rodrigues, A. A., Macedo, M. N., Silvério, D. V., Maracahipes, L., Coe, M. T., Brando, P. M., Shimbo, J. Z., Rajão, R., Soares‐Filho, B., & Bustamante, M. M. C. 2022 Cerrado deforestation threatens regional climate and water availability for agriculture and ecosystems. Global Change Biology, 28(22), 6807–6822. https://doi.org/10.1111/gcb.16386

Sánchez-Cuevas, M. C., & Salaverría, J. L. 2004 Control de la oxidación y la contaminación en el cultivo in vitro de fresa (Fragaria X ananassa Duch. Revista UDO Agrícola 4(1), 21-26.

Salgotra, R. K., & Chauhan, B. S. 2023 Genetic Diversity, Conservation, and Utilization of Plant Genetic Resources. Genes, 14(1), 174. https://doi.org/10.3390/genes14010174

San José, M. C., Cernadas, M. J., & Janeiro, L. V. 2021 Optimization of Micropropagation Protocols in Some Woody Plants Using Meta-topolin. Em Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture (p. 221–240 Springer Singapore. https://doi.org/10.1007/978-981-15-9046-7_16

Santini, N. S., & Miquelajauregui, Y. 2022 The Restoration of Degraded Lands by Local Communities and Indigenous Peoples. Frontiers in Conservation Science, 3. https://doi.org/10.3389/fcosc.2022.873659

Santos, R. N. M. dos. 2003 Indicadores estratégicos em ciência e tecnologia: refletindo a sua prática como dispositivo de inclusão/exclusão. Transinformação, 15(spe), 129–140. https://doi.org/10.1590/S0103-37862003000500007

Scarano, F. R., & Martinelli, G. 2010 Brazilian List of Threatened Plant Species: Reconciling Scientific Uncertainty and Political Decision-Making. Natureza & Conservação, 08(01), 13–18. https://doi.org/10.4322/natcon.00801002

Schatz, G. E. 2009 Plants on the IUCN Red List: setting priorities to inform conservation. Trends in Plant Science, 14(11), 638–642. https://doi.org/10.1016/j.tplants.2009.08.012

Shahzad, A., Parveen, S., & Fatema, M. 2011 Development of a regeneration system via nodal segment culture in Veronica anagallis-aquatica L. – an amphibious medicinal plant. Journal of Plant Interactions, 6(1), 61–68. https://doi.org/10.1080/17429141003646675

Shahzad, A., Sharma, S., Parveen, S., Saeed, T., Shaheen, A., Akhtar, R., Yadav, V., Upadhyay, A., & Ahmad, Z. 2017 Historical Perspective and Basic Principles of Plant Tissue Culture. Em Plant Biotechnology: Principles and Applications (p. 1–36 Springer Singapore. https://doi.org/10.1007/978-981-10-2961-5_1

Sharma., H. 2017 Role Of Growth Regulators In Micropropagation Of Woody Plants-A Review. International Journal of Advanced Research, 5(2), 2378–2385. https://doi.org/10.21474/IJAR01/3421

Sidone, O. J. G., Haddad, E. A., & Mena-Chalco, J. P. 2016 A ciência nas regiões brasileiras: evolução da produção e das redes de colaboração científica. Transinformação, 28(1), 15–32. https://doi.org/10.1590/2318-08892016002800002

Silva, H. F. de J., Asmar, S. A., Oliveira, R. C. de, Melo, B. De, Luz, J. M. Q., & Pasqual, M. 2016 In vitro establishment and early development of barueiro (Dipteryx alata Vogel Semina: Ciências Agrárias, 37(4), 1779. https://doi.org/10.5433/1679-0359.2016v37n4p1779

Silva, T. D., Chagas, K., Batista, D. S., Felipe, S. H. S., Louback, E., Machado, L. T., Fernandes, A. M., Buttrós, V. H. T., Koehler, A. D., Farias, L. M., Santos, A. F., Silva, P. O., & Otoni, W. C. 2019 Morphophysiological in vitro performance of Brazilian ginseng (Pfaffia glomerata (Spreng.) Pedersen) based on culture medium formulations. In Vitro Cellular & Developmental Biology - Plant, 55(4), 454–467. https://doi.org/10.1007/s11627-019-10003-9

Silveira, S. S., Cordeiro-Silva, R., Degenhardt-Goldbach, J., & Quoirin, M. 2016 Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments. Brazilian Journal of Biology, 76(3), 656–663. https://doi.org/10.1590/1519-6984.23714

Song, W., Song, Y., Liu, X., Zhang, X., Xin, R., Duan, S., Guan, S., & Sun, X. 2023 Improvement of Culture Conditions and Plant Growth Regulators for In Vitro Callus Induction and Plant Regeneration in Paeonia lactiflora Pall. Plants, 12(23), 3968. https://doi.org/10.3390/plants12233968

Souza Coccorese Conceição, I., Portela Carmo, L., & Lima-Brito, A. 2021 Costreductionin the micropropagation of Solanum lycopersicumL. var. cerasiforme. COLLOQUIUM AGRARIAE, 17(3), 12–20. https://doi.org/10.5747/ca.2021.v17.n3.a435

Superpowered Science: Charting China’s Research Rise. Nature, v. 593, n. 7860, p. S4–S5, 2021. https://doi.org/10.1038/d41586-021-01403-2

Suwal, M. M., Lamichhane, J., & Gauchan, D. P. 2020 Regeneration Technique of Bamboo Species through Nodal Segments: A Review. Nepal Journal of Biotechnology, 8(1), 54–68. https://doi.org/10.3126/njb.v8i1.30209

Tambarussi, E. V., Rogalski, M., Nogueira, F. T. S., Brondani, G. E., De Martin, V. de F., & Carrer, H. 2015 Influence of antibiotics on indirect organogenesis of Teak. Annals of Forest Research, 58(1), 1. https://doi.org/10.15287/afr.2015.345

Tambarussi, E. V., Rogalski, M., Galeano, E., Brondani, G. E., Martin, V. de F. de, Silva, L. A. da, & Carrer, H. 2017 Efficient and new method for Tectona grandis in vitro regeneration. Crop Breeding and Applied Biotechnology, 17(2), 124–132. https://doi.org/10.1590/1984-70332017v17n2a19

Tran, Nam. 6 plant tissue culture media for beginners. 27 Sep. 2023. Lab Associates. Available from: https://labassociates.com/6-plant-tissue-culture-media-for-beginners. Acesso em: 3 out. 2024.

Thorpe, T. A. 2007 History of plant tissue culture. Molecular Biotechnology, 37(2), 169–180. https://doi.org/10.1007/s12033-007-0031-3

Thorpe, Trevor A. 1994 Morphogenesis and regeneration. In: Plant cell and tissue culture. Dordrecht: Springer Netherlands, 17-36.

Tian, Daike. 2008) Container production and post-harvest handling of lotus (Nelumbo) and micropropagation of herbaceous peony (Paeonia J. Environ. Hort. 27(2):79–79.

Uchida, N., & Torii, K. U. 2019 Stem cells within the shoot apical meristem: identity, arrangement and communication. Cellular and Molecular Life Sciences, 76(6), 1067–1080. https://doi.org/10.1007/s00018-018-2980-z

Usenbekov, B., Amirova, A., Zeinalov, Z., Meldebekova, A., Mynbayeva, D., Berkimbay, Kh., & Kurbangaliyeva, T. 2024 Creation of rice doubled haploids with low amylose content using in vitro anther culture. Brazilian Journal of Biology, 84. https://doi.org/10.1590/1519-6984.284946

Vadell, E., de-Miguel, S., & Pemán, J. 2016 Large-scale reforestation and afforestation policy in Spain: A historical review of its underlying ecological, socioeconomic and political dynamics. Land Use Policy, 55, 37–48. https://doi.org/10.1016/j.landusepol.2016.03.017

Van Eck, N. J., & Waltman, L. 2014 Visualizing Bibliometric Networks. Em Measuring Scholarly Impact (p. 285–320 Springer International Publishing. https://doi.org/10.1007/978-3-319-10377-8_13

Vidal-Couto, D. B., Brito, C. R. de, Andrade, I. L. M. M., Cerqueira, A. F., Reis, I. P., Tomasini, S. L. V., Dalmolin, Â. C., & Mielke, M. S. 2023 Tree species used in urban forestry in Brazil: a scientometric review. Rodriguésia, 74. https://doi.org/10.1590/2175-7860202374047

VISWANATH, Syam. 2013 Dendrocalamus stocksii (Munro.): A potential multipurpose bamboo species for Peninsular India. Institute of Wood Science and Technology.

Vitt, P., Finch, J., Barak, R. S., Braum, A., Frischie, S., & Redlinski, I. 2022 Seed sourcing strategies for ecological restoration under climate change: A review of the current literature. Frontiers in Conservation Science, 3. https://doi.org/10.3389/fcosc.2022.938110

Xu, J., Xiao, P., Li, T., & Wang, Z. 2022 Research Progress on endangered plants: a bibliometric analysis. Biodiversity and Conservation, 31(4), 1125–1147. https://doi.org/10.1007/s10531-022-02392-y

Xue, Y., Hiti-Bandaralage, J. C. A., & Mitter, N. 2023 Micropropagation of Duboisia Species: A Review on Current Status. Agronomy, 13(3), 797. https://doi.org/10.3390/agronomy13030797

Yancheva, S., & Kondakova, V. 2016 Plant Tissue Culture Technology: Present and Future Development (p. 1–26 https://doi.org/10.1007/978-3-319-32004-5_16-1

Yang, Q., Yang, D., Li, P., Liang, S., & Zhang, Z. 2021 Resilient City: A Bibliometric Analysis and Visualization. Discrete Dynamics in Nature and Society, 2021, 1–17. https://doi.org/10.1155/2021/5558497

Downloads

Publicado

2025-02-18

Como Citar

SILVA, Gabriel Alves de Souza da; ROCHA, Tainá Teixeira; HERRERA, Raírys Cravo. Micropropagação de Espécies Lenhosas Nativas: Análise do Conhecimento Científico e Perspectivas para a Conservação. Fronteira: Journal of Social, Technological and Environmental Science, [S. l.], v. 14, n. 1, p. 267–298, 2025. DOI: 10.21664/2238-8869.2025v14i1.p267-298. Disponível em: https://periodicos.unievangelica.edu.br/index.php/fronteiras/article/view/7816. Acesso em: 21 fev. 2025.