Glyceolin and phaseolin induction in soybean and beans as a function of biocontrol agents application

Abstract

Induction of plant resistance is a tool that can be incorporated into the integrated management of diseases of cultivated plants. In this way, this work aims to evaluate the elicitor action of biocontrol agents in the induction of glyceolin. In this study were used concentrations about 0; 0.5; 1; 1.5 and 2% diluted in water; spore suspension of Trichoderma asperellum BV10, Bacillus subtilis BV02 and Bacillus amyloliquefaciens BV03 microorganisms. To determine the glyceolin, cotyledons were grown in sand, weighed and cut in longitudinal section on the lower surface. Subsequently, these were deposited 50 μL of the concentrations in the cuts and the extraction performed in H₂O and concentration determined by absorbance at wavelength 285nm. The results were submitted to analysis of variance and compared by the regression test (p <0.05). When measuring the glyceolin in soybean cotyledons, there was an increase in the accumulation of this phytoalexins as the concentration of B. subtilis BV02 cells increased. The 4% concentration of B. subtilis BV02 promoted 237% more glyceolin accumulation in soybean cotyledons than the control. The accumulation of glyceolin was increased as the concentration of T. asperellum BV10 cells increased, so that 4% concentration promoted 228% more glycerol accumulation than the control. Increasing the concentration of Bacillus amyloliquefaciens BV03 cells increased the accumulation of this phytoalexins. The 4% concentration promoted 129% more accumulation of glycerol in soybean cotyledons than the control. The microorganisms T. asperellum, B. subtilis and B. amyloliquefaciens are indicated as activators of resistance mechanisms of soybean plants.