Antimicrobial Activity an Physicochemical Characterization of Extracts and Fractions of Rosmarinus officinalis and Origanum vulgare
DOI:
https://doi.org/10.21664/2238-8869.2022v11i1.p8-30Palavras-chave:
atividade antimicrobiana, O. vulgare, extratos de plantas, R. officinalisResumo
Rosmarinus officinalis L. (Lamiaceae) é um arbusto lenhoso com sabor agridoce, utilizado em batatas fritas, caldos, sobremesas, biscoitos, compotas, saladas de frutas e marmeladas. Origanum vulgare L. (Lamiaceae) é um pequeno arbusto utilizado em carnes, saladas, sopas, arroz, batata e ovos cozidos, bem como em óleos perfumados e aromatizantes. Ambos possuem propriedades medicinais e aromáticas. Os objetivos deste estudo foram: realizar a triagem fitoquímica do pó das folhas; a caracterização físico-química dos extratos etanólicos brutos de R. officinalis e O. Vulgare; determinar o teor de polifenóis, taninos e flavonóides; quantificar o ácido rosmarínico; avaliar a atividade antimicrobiana do ácido rosmarínico, extratos, frações de R. officinalis e O. vulgare contra cepas clínicas e de origem alimentar. A atividade antimicrobiana foi avaliada pelo método de microdiluição em caldo. As frações hexano e diclorometano de R. officinalis e O. vulgare e ácido rosmarínico exibiram boa atividade inibitória (CIM <100 μg/mL) contra algumas bactérias Gram-positivas e isolados de alimentos. A fração de acetato de etila de R. officinalis (MIC = 62,5 μg/mL), extrato etanólico bruto (CIM = 15,6 μg/mL), fração hexano (CIM = 62,5 μg/mL), fração diclorometano (CIM = 31,2 μg/mL) e a fração aquosa (CIM = 62,5 μg/mL) de O. vulgare e ácido rosmarínico (CIM = 31,2 μg/mL) apresentaram boa atividade inibitória contra Cryptococcus. Essas frações têm potencial promissor no controle de patógenos alimentares e podem ser utilizadas no futuro como alternativa conservante natural na indústria.
Referências
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Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X 2014. Secondary metabolites isolation in natural products chemistry: Comparison of two semi preparative chromatographic techniques (high-pressure liquid chromatography and high performance thin-layer chromatography). J Chromatogr A 1325: 256-260.
Erkan N, Ayranci G, Ayranci E 2008. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 110:76-82.
Falco E, Mancini E, Roscigno G, Mignola E, Taglialatela-Scafati O, Senatore F 2013. Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp. vulgare L. under different growth conditions. Molecules 18: 14948-14960.
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Frescura VS, Boligon AA, Athayde ML, Andriolo JL, Tedesco SB 2013. Compostos fenólicos em extratos de Rosmarinus Officinalis L. sob cultivo fora do solo. Enciclopédia Biosfera, Centro Científico Conhecer – Goiânia. 9: 755-761.
Gachkar L, Yadegari D, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I 2007. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem 102: 898 904.
Gohari AR, Saeidnia S, Shahverdi AR, Yassa N, Malmir M, Mollazade K, Naghinejad AR 2009. Phytochemistry and antimicrobial compounds of Hymenocrater calycinus. Eur Asian J Bio Sciences. 3: 64-68.
Gosslau A, Li S, Ho C, Chen KY, Rawson NE 2011. The importance of natural product characterization in studies of their anti-inflammatory activity. Mol Nutr Food Res 55: 74-82.
Grevsen K, Fretté XC, Christensen LP 2009. Content and composition of volatile terpenes, flavonoids and phenolic acids in greek oregano (Origanum vulgare L. ssp. hirtum) at different development stages during cultivation in cool temperate climate. Eur J Hortic Sci 74: 193-203.
Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP 2002. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 97: 1027-1031.
Janampa E, Perez H, Mauricio F, Alvítez‑Temoche D, Mauricio‑Vilchez C, Gallo W, Mayta‑Tovalino F 2020. Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An in vitro comparative study. European J Gen Dent 9: 152-156.
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2022-04-29
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CHAUL, Luiza Toubas; ALVES, Virgínia Farias; SÁ, Stone de; OLIVEIRA, Leandra de Almeida Ribeiro; FIUZA, Tatiana de Sousa; TORRES, Ieda Maria Sapateiro; CONCEIÇÃO, Edemilson Cardoso da; PAULA, José Realino de. Antimicrobial Activity an Physicochemical Characterization of Extracts and Fractions of Rosmarinus officinalis and Origanum vulgare. Fronteira: Journal of Social, Technological and Environmental Science, [S. l.], v. 11, n. 1, p. 8–30, 2022. DOI: 10.21664/2238-8869.2022v11i1.p8-30. Disponível em: https://periodicos.unievangelica.edu.br/index.php/fronteiras/article/view/4029. Acesso em: 24 abr. 2024.
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